Search results for " DNA barcoding"
showing 6 items of 6 documents
Re-description of Orchestia stephenseni Cecchini, 1928: designation of neotype and senior synonym to Orchestia constricta A. Costa, 1853 (Crustacea: …
2016
The beach flea Orchestia stephenseni was originally described by Cecchini (1928), and successively by Karaman (1973). The description of this species will be herein revised by focusing on the variation of the g nathopod 2 in males, as detected during its growth period. An analysis of DNA Barcoding was performed to support the assignment of the taxonomic species to five morphotypes. As the type specimen has not yet been designated, a neotype is assigned. The name of the species is here presented as a valid name as it satisfies the requirements of a Reversal of the Principle of Priority: Orchestia stephenseni takes precedence over the objective synonym Orchestia constricta A. Costa, 1853, in …
Tassonomia integrata per lo studio della biodiversità vegetale
2010
Diversi marcatori plastidiali sono stati testati su varie specie della flora vascolare spontanea al fine di valutarne il potere discriminante come DNA barcoding, da integrare nell'identificazione tassonomica con i dati morfologici, cariologici, fenologici e biogeografici.
Evaluation of criteria for species delimitation of bagworm moths (Lepidoptera: Psychidae)
2014
Accurate identification of species is fundamental for biological research and necessary for species conservation. DNA bar- coding is particularly useful when identification using morphological characteristics is laborious and/or unreliable. However, bar- codes for species are dependent on the availability of reference sequences from correctly identified specimens. The traditional use of morphology to delimit the species boundaries of Finnish bagworm moths (Lepidoptera: Psychidae: Naryciinae: Dahliciini) is contro- versial because there is overlap in their morphological characteristics. In addition, there are no suitable molecular markers. We veri- fied the delimitation of seven out of eight…
A contribution to the characterization of Ruppia drepanensis (Ruppiaceae), a key species of threatened Mediterranean wetlands
2021
To elucidate the taxonomic status of Ruppia drepanensis Tineo ex Guss. (Alismatales, Ruppiaceae), we performed morphological analysis and DNA barcoding of historical materials (including the lectotype) and fresh samples (including those from a recently discovered population near the locus classicus in Sicily, Italy). We conclude that R. drepanensis is a separate species, closely related to R. spiralis L. ex Dumort., that occurs in temporary inland waters from the western to central sectors of the Mediterranean region. We also highlight the importance of vouchers and the need to link molecular investigations to field, ecological, and morphological investigations.
Assessing the use of the mitochondrial cox1 marker for use in DNA barcoding of red algae (Rhodophyta)
2011
The red algae, a remarkably diverse group of organisms, are difficult to identify using morphology alone. Following the proposal to use the mitochondrial cytochrome c oxidase subunit 1 (cox1) for DNA barcoding animals, we assessed the use of this, ne in the identification of red algae using 48 samples plus 31 sequences obtained from GenBank. The data set spanned six gene orders of red algae: the Bangiales, Ceramiales, Corallinales, Gigartinales, Gracilariales and Rhodymeniales. The results indicated that species could be discriminated. Intraspecific variation was between 0 and 4 bp over 539 bp analyzed except in Mastocarpus stellatus (0-14 bp) and Gracilaria gracilis (0-11 bp). Cryptic dive…
An integrated approach to the study of Hypericum occurring in Sicily
2020
An integrated approach to the study of taxa of the genus Hypericum occurring in Sicily is proposed. The results of morphological, biochemical, and molecular analyses are combined to better assess the relationships between the species investigated and test the suitability of DNA barcoding technique in the discrimination of these taxa. For the name Hypericum aegypticum subsp. webbii (Spach) N. Robson a lectotype is designated. For Hypericum triquetrifolium Turra a lectotype and a supporting epitype are designated. The presence of Hypericum perforatum L. subsp. perforatum is excluded from Sicily and the previous reports have to be referred to H. perforatum subsp. veronense (Schrank) Ces. Hyper…